Neurotransmitter sensors and methods of using the same

Details Neurotransmitter sensors and methods of using the same Neurotransmitter sensors and methods of using the same

C - Chemistry, Metallurgy

07

H

C07H 21/04 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 5/16 (2006.01) C12N 15/62 (2006.01) C12N 15/70 (2006.01) C12N 15/79 (2006.01)

Patent

CA 2584116

Neurotransmitter biosensors are disclosed, including YbeJ-based glutamate binding biosensors, comprising a neurotransmitter binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding neurotransmitter. Such biosensors are useful for the detection of neurotranmitter concentrations in vivo and in culture.

Biocapteurs neurotransmetteurs, comprenant des biocapteurs de liaison de glutamate utilisant YbeJ, qui comprennent un domaine de liaison neurotransmetteur conjugué à un donneur et des fractions fluorescentes permettant la détection et la mesure du transfert d'énergie de résonance de fluorescence lors de la liaison du neurotransmetteur. De tels biocapteurs sont utilisés pour la détection de concentrations de neurotransmetteurs in vivo et en culture.

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