Neutral buoyancy glass-surface microcarrier for growth of...

Details Neutral buoyancy glass-surface microcarrier for growth of... Neutral buoyancy glass-surface microcarrier for growth of...

C - Chemistry, Metallurgy

12

N

195/40, 195/34.1

C12N 5/00 (2006.01) C12N 11/14 (2006.01)

Patent

CA 1191096

Abstract of the Disclosure A microcarrier for anchorage-dependent cell culturation comprising, and preferably consisting essentially of, a spherical substrate of polymeric material having a bulk density of about 1 g/cc so as to be substantially buoyant in an aqueous culture medium, and a thin (less than 1 µm) coating layer of silicate glass. The silicate glass coating layer is preferably applied to a polymeric precursor in an rf sputtering operation. An intermediate coating layer of magnetic material may be deposited prior to the silicate glass layer, so that the microcarriers may be readily removed from culture media by suitable subjection to a magnetic field.

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