Neutral cellulase catalytic core and method of producing same

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/42 (2006.01) A23K 1/165 (2006.01) C11D 3/386 (2006.01) C12N 15/55 (2006.01) D06M 16/00 (2006.01) D21C 5/00 (2006.01)

Patent

CA 2591221

The present invention relates to the cloning and high level expression of novel cellulase proteins or derivatives thereof in the in a host cell. Further aspects of the present invention relate to transformants that express the novel cellulases, and expression vectors comprising the DNA gene fragments or variants thereof that code for the novel cellulases derived from Actinomycete using genetic engineering techniques. The present invention is also directed to novel cellulase compositions and methods of use therefore in industrial processes. In particular, the present invention is related to treating textiles with a novel cellulase derived from Actinomycete spp. The present invention also relates to the use of cellulase derived from Actinomycete spp. to enhance the digestibility of animal feed, in detergents, in the treatment of pulp and paper and in the production of starch and treatment of by-products thereof.

L'invention concerne le clonage et l'expression à un niveau élevé de protéines cellulase ou de dérivés correspondants dans une cellule hôte. Sous d'autres aspects, l'invention concerne des transformants qui expriment ces protéines, et des vecteurs d'expression qui renferment des fragments géniques d'ADN ou des variants correspondants pour le codage des protéines en question, extraites d'actinomycète par génie génétique. On décrit aussi des compositions de cellulase et des procédés d'utilisation correspondants dans le domaine industriel. L'invention concerne en particulier le traitement de textiles par le biais d'une cellulase de ce type, issue d'actinomycète spp. On décrit enfin l'utilisation de cellulase issue d'actinomycète spp. pour améliorer la digestibilité d'aliments destinés aux animaux, l'utilisation de cette cellulase dans des détergents, et son utilisation dans le traitement de la pâte et du papier, ainsi que dans la production d'amidon et pour le traitement de sous-produits correspondants.

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