Neutrophil adherence-inhibiting substance of human origin...

C - Chemistry – Metallurgy – 07 – K

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C07K 14/47 (2006.01) A61K 38/17 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2089868

47 ABSTRACT OF THE DISCLOSURE A novel human neutrophil adherence-inhibiting substance having the following properties (a) to (g) was identified. (a) It is localized in a platelet granule fraction but released extracellularly in response to a platelet activation stimulus. (b) It has a function to inhibit neutrophil adherence to substrata. (c) It has a function to inhibit neutrophil spreading reaction. (d) Its molecular weight is 2,600 ? 260 kd when measured by gel filtration. (e) Its molecular weight is 340 ? 34 kd and/or 190 ? 19 kd when measured by SDS-PAGE under reducing condition. (f) It is inactivated by trypsin treatment. (g) It contains an amino acid sequence "Pro Glu Arg Glu Gln Xaa Lys" as a part thereof. This substance is useful as an active ingredient of a pharmaceutical composition for the treatment of inflammatory diseases such as rheumatoid arthritis, systemic lupus erythematosus, dermatomyositis, rejection reaction, reperfusion disorder, chronic nephritis, chronic hepatitis, asthma and the like.

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