C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/32 (2006.01) A01H 5/00 (2006.01) A01N 37/18 (2006.01) A01N 43/50 (2006.01) A01N 57/16 (2006.01) A01N 63/00 (2006.01) C07K 14/325 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2160131
Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis spp., against Pyralidae such as Ostrinia nubilalis, against Gelechiidae such as Phthorimaea operculella, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystrals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.
Quatre nouvelles souches de Bacillus thuringiensis, qui sont déposées à la BCCM-LMG (Belgian Coordinated Collections of Microorganisms), sous les numéros d'enregistrement LMG P-12592, LMG P-12593, LMG P-12594 et LMG P-13493, produisent de nouvelles protéines cristallines pendant la sporulation, qui sont toxiques pour les lépidoptères, plus particulièrement les noctuidés tels que Spodoptera spp. et Agrotis spp., les pyralides tels que Ostrinia nubilalis, les géléchiidés tels que Phthorimaea operculella et les yponomentidés tels que Plutella xylostella et qui sont codés par un nouveau gène. Les protéines cristallines contiennent des protoxines, qui peuvent produire une toxine comme produit de digestion trypsine. Une plante, dont le génome est transformé avec une séquence ADN qui vient de l'une des souches et qui code sa toxine respective, est résistante aux lépidoptères. Chaque souche, elle-même ou ses cristaux, ses protéines cristallines, sa protoxine ou sa toxine, peut être utilisée comme ingrédient actif dans une composition insecticide pour combattre les lépidoptères.
Jansens Stefan
Lambert Bart
Peferoen Marnix
Van Aarssen Roel
Van Audenhove Katrien
Fetherstonhaugh & Co.
Plant Genetic Systems N.v.
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