New brucella antigens, recombinant polypeptides, nucleic...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/31 (2006.01) A61K 39/10 (2006.01) C07K 14/23 (2006.01) C07K 16/12 (2006.01) C12N 1/21 (2006.01) C12Q 1/04 (2006.01) C12Q 1/68 (2006.01) G01N 33/569 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2263791

The present invention relates to isolated and pure Brucella antigens, to nucleic acids encoding said antigens, as well as to diagnostic methods and kits using said antigens and nucleic acids for detecting Brucella infection in human and cattle. The invention also relates to recombinant polypeptides, a process for preparing the same and their use in methods and kits for the diagnosis of Brucella infection. The invention also relates to the use of said isolated antigens, or said recombinant polypeptides, or said nucleic acids as an active principle of a vaccine composition against Brucella infection. The invention relates also to a vaccine composition comprising a recombinant Brucella strain, specifically deleted for the gene(s) encoding said antigen(s).

La présente invention concerne des antigènes purs et isolés de la Brucella, des acides nucléiques codant lesdits antigènes, ainsi que des procédés et trousses diagnostiques faisant appel à ces antigènes et acides nucléiques pour détecter l'infection par Brucella chez l'homme et le bétail. L'invention se rapporte également à des polypeptides recombinés, à leur procédé de préparation et à leur utilisation dans des procédés et des matériels de diagnostic de l'infection par Brucella. On décrit également l'utilisation de ces antigènes isolés, ou de ces polypeptides recombinés, ou de ces acides nucléiques comme principe actif d'une composition de vaccin contre l'infection par Brucella. On décrit également une composition de vaccin comprenant une souche recombinée de Brucella, dans laquelle le(s) gène(s) codant lesdits antigènes a/ont été spécifiquement supprimé(s).

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