New cationic peptides of the dermaseptin family isolated...

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 7/08 (2006.01) C07K 14/435 (2006.01)

Patent

CA 2453112

This invention is related to anti-microbial peptides having the same amino acid sequence and at least the same anti-microbial activity as those of Phylloseptins which are isolated from the skin of Phyllomedusa hypochondrialis. The peptides did not have any structural homology with another known peptide. The 19-residue anti-microbial peptides are cationic and based on their primary structure, all peptides can be fitted to an amphiphilic a-helix. The peptide masses analyzed by mass spectrometry were in the range of 1.9 to 2.0 kDa.These peptides can be used in compositions for retarding plant pathogens and for protecting plants from pathogens, comprising at least one antibiotic peptide and an agriculturally acceptable carrier. Another embodiment of the invention is to provide therapeutic compositions suitable for human, veterinary, or pharmaceutical use, comprising one or more of the peptides released in this invention and an adequate pharmacological carrier.

Cette invention concerne des peptides anti-microbiens présentant la même séquence d'acides aminés et au moins la même activité anti-microbienne que celles des Phylloseptines qui sont isolées à partir de la peau des Phyllomedusa hypochondrialis . Les peptides isolés ne présentent aucune homologie structurelle avec un autre peptide connu. Les peptides anti-microbiens à 19 résidus sont cationiques et basés sur leur structure primaire, tous les peptides pouvant être adaptés à une alpha -hélice amphiphilique. Les masses peptidiques analysées par spectrométrie de masse sont comprises entre 1,9 et 2,0 kDa. Ces peptides peuvent être utilisés dans des compositions servant à retarder des agents pathogènes de plantes et à protéger les plantes contre ces agents pathogènes, et renfermant au moins un peptide antibiotique et un transporteur acceptable sur le plan agricole. Un autre mode de réalisation de cette invention concerne des compositions thérapeutiques aptes à une utilisation humaine, vétérinaire ou pharmaceutique, renfermant un ou plusieurs peptides décrits par cette invention ainsi qu'un transporteur pharmacologique adéquat.

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