New escherichia coli host/vector system based on...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/70 (2006.01) C07K 14/56 (2006.01) C12N 15/12 (2006.01) C12N 15/64 (2006.01) C12N 15/65 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2274982

A minimal prokaryotic expression vector which cannot be homologously recombined with the genome of prokaryotic organisms containing: e) an origin of replication, f) an auxotrophy marker gene, g) a promoter which is functionally active in prokaryotes and h) a foreign gene to be expressed which is under the control of the said promoter is particularly suitable for antibiotic-free selection.

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