New expression tools for multiprotein applications

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/866 (2006.01) A01K 67/027 (2006.01) A61K 31/7088 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2558313

The present invention relates to polynucleotides for multigene applications comprising a novel functional arrangement, as well as vectors, host cells, and recombinant animals comprising said polynucleotides. In addition, the present invention is directed to methods for generating multigene expression cassettes, methods for producing multiprotein complexes in vitro and in vivo, and methods for producing a vaccine. Furthermore, the present invention encompasses methods for screening protein complex interactions or modifications of proteins, and methods for the in vitro or in vivo screening of candidate compounds capable of protein complex interactions or modifications of proteins or capable of inhibiting protein complex interactions or inhibiting modifications of proteins. Also, the present invention relates to the use of the polynucleotides, vectors, host cells or recombinant animals of the invention for (i) preparing a medicament for gene therapy, for (ii) the recombinant production of multiprotein complexes, (iii) for producing a vaccine, or (iv) for screening compounds of interest. Last but not least, the invention is directed to a kit of parts comprising at least a polynucleotide, a vector, and/or a host cell according to the invention.

La présente invention concerne des polynucléotides pour applications multigéniques, comprenant un nouvel agencement fonctionnel, ainsi que des vecteurs, des cellules hôtes et des animaux recombinants comprenant lesdits polynucléotides. En outre, cette invention concerne des procédés pour générer des cassettes d'expression multigénique, des procédés pour produire des complexes multiprotéiques in vitro et in vivo ainsi que des procédés pour produire un vaccin. De plus, ladite invention concerne des procédés pour cribler des interactions de complexes protéiques ou des modifications de protéines ainsi que des procédés pour le criblage in vitro ou in vivo de composés candidats capables d'induire des interactions de complexes protéiques ou des modifications de protéines ou capables d'inhiber des interactions de complexes protéiques ou d'inhiber des modifications de protéines. La présente invention concerne également l'utilisation de ces polynucléotides, vecteurs, cellules hôtes ou animaux recombinants (i) pour préparer un médicament pour la thérapie génique, (ii) pour la production recombinante de complexes multiprotéiques, (iii) pour produire un vaccin ou (iv) pour cribler des composés d'intérêt. Ladite invention concerne, enfin et surtout, une trousse contenant au moins un polynucléotide, un vecteur et/ou une cellule hôte selon l'invention.

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