C - Chemistry – Metallurgy – 08 – F
Patent
C - Chemistry, Metallurgy
08
F
C08F 20/56 (2006.01) B01J 20/26 (2006.01) B01J 20/291 (2006.01) C08F 20/58 (2006.01) G01N 27/447 (2006.01)
Patent
CA 2125136
Matrices for electrokinetic and chromatographic separations, based on a unique class of mono- and disubstituted (on the amido nitroagen) acrylamides (e.g., acrylamido-N-ethoxy ethanol, acrylamido-N, N-di-ethoxy ethanol) offer the following ad- vantages: a) strong resistance to alkaline hydrolysis (most zone separations occurring at basic pH values); b) a high hydrophilici- ty and c) greater porosity, due to the larger M r value of the monomers. Such matrices can be prepared as 'chemically cross- linked' gels or as 'physical gels', the latter consisting of'strings' of monofunctional monomer in the absence of cross-linker, at concentrations above the entanglement threshold. It is moreover possible to further increment the gel porosity by polym- erizing it in presence of laterally-aggregating agents (e.g., polyethylene glycol of M r 6-20000 Da), thus obtaining matrices which allow better resolution of large M r proteins and of nucleic acid fragments; by polymerizing the matrix in presence of a gradient of the laterally-aggregating agent, it is possible to obtain a porosity gradient even at constant per cent of monom- ers in the gel. Finally, photopolymerization (e.g., with methylene blue and a redox couple, sodium toluenesulfinate and di- phenyliodonium chloride and light), instead with the standard red/ox catalysts, prevents formation of N-oxides of amines present in the polymerizing solution.
Chiari Marcella
Righetti Pier Giorgio
Kirby Eades Gale Baker
Righetti Pier Giorgio
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