C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/60 (2006.01) C07C 255/00 (2006.01) C07K 19/00 (2006.01) C12N 9/88 (2006.01) C12N 15/52 (2006.01) C12N 15/62 (2006.01) C12P 13/00 (2006.01)
Patent
CA 2366135
New genes containing a DNA sequence coding for hydroxynitrile lyase, which genes can be prepared via a primer combination based on the DNA sequence of of the 5'-region of the mdl genes from Prunus serotina and from Prunus amygdalus and/or a primer 2 based on the 3'-region of the DNA sequences of one of the hydroxynitrile lyase isoenzymes from Prunus serotina or from Prunus amygdalus, subsequent amplification with a DNA polymerase using a DNA from organisms, containing genes coding for hydroxynitrile lyase, as templates and cloning, and also recombinant proteins which can be prepared in suitable host cells by heterologous expression of the DNA sequence of said HNL genes, and proteins and fusion proteins derived therefrom and use of said proteins for preparing (R)-or (S)-cyanohydrins.
Bona Rodolfo
Dreveny Ingrid
Glieder Anton
Kratky Christoph
Mayrhofer Herbert
Dsm Fine Chemicals Austria Nfg Gmbh & Co Kg.
Riches Mckenzie & Herbert Llp
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