C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/02 (2006.01) C12N 9/04 (2006.01) C12P 7/62 (2006.01) C12P 41/00 (2006.01)
Patent
CA 2117482
An enzyme designated as ketonic ester reductase capable of being used in the NADH-dependent enzymatic reaction of .beta.. y and .delta. ketonic acid esters into the corresponding optically active .beta., y and .delta. hydroxycarboxylic acid esters can he isolated from strains of Candida parapsilosis. Yarrowinia cellobiosa, Rhodococcus erythropolis or Pseudomonas acidovorans, preferably cultivated on a long-chain alkane and/or alkane acid-containing culture medium, appropriately in the presence of an inductor. An usable enzyme preparation can be recovered by fractionated PEG-precipitation from the cell raw extract; high specific activities (for example 1855 U/mg) may then be obtained by chromatographic purification. The enzyme is characterized by a wide substrate spectrum. Not only (possibly substituted) so-called ketonic esters are accepted, but also a number of other oxo-compounds, among which diketones, (possibly substituted, in particular halogenated) aliphatic, alicyclic and aromatic ketones, as well as ketoacetals and aldehydes. The S-enamiomer-forming reduction is supplemented by the possibility to recover R-enanuomers from racemates by oxidizing the S-enantiomer and separating the oxo-compound.
Kula Maria-Regina
Peters Jorg
Forschungszentrum Julich Gmbh
Smart & Biggar
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