New mutants of formate dehydrogenase from candida boidinii,...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/53 (2006.01) C12N 9/02 (2006.01) C12P 13/04 (2006.01)

Patent

CA 2253021

The invention relates to new mutants of formate dehydrogenase (FDH) from Candida boidinii, new gene sequences encoding these, and use of the new formate dehydrogenases. The wild type FDH used hitherto in the industrial process for preparing amino acids becomes inactive after time. New mutants of this wild type FDH have been produced by targeted mutagenesis of a recombinant FDH from E. coli. The new mutants can be used in an enzymatic process for preparing chiral compounds.

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