C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2471230
The present invention relates to new primers for the detection of any of three important bacterial indicator groups used in food microbiology and two virulence factors, which are associated with the aetiology of several types of watery and bloody human diarrhoea. It also provides a method for subtyping the two virulence factors. Furthermore, the present invention also relates to use of the primers, as well as use of the primers in a method which enables this detection, as well as the detection of new emerging pathogenic bacteria. The bacterial indicator group are identified by using primers specific for the aa (6') - Ii, eep, gadA and GadB genes. The virulence factors are identified by using primers specific for the stx and eae genes.
La présente invention concerne des nouvelles amorces destinées à la détection de trois groupes d'indicateurs bactériens importants utilisés en microbiologie alimentaire et de deux facteurs de virulence, lesquels sont associés à l'étiologie de divers types de diarrhées humaines aqueuses et sanglantes. L'invention concerne en outre une méthode destinée au sous-typage de ces deux facteurs de virulence. Par ailleurs, la présente invention se rapporte à l'utilisation desdites amorces dans une méthode permettant cette détection, ainsi qu'à la détection de nouvelles bactéries pathogènes émergentes.
Angles D'auriac Marc B.
Goudreau Gage Dubuc
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