New ultra-sensitive chemiluminescent substrates for enzymes...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/34 (2006.01) C12Q 1/00 (2006.01) C12Q 1/28 (2006.01) G01N 33/52 (2006.01)

Patent

CA 2562684

New chemiluminescent compounds, stable in aqueous buffers, for use in biological assaying include acridane-based compounds and (1,2)-dioxetanes. Among the new acridane~based compounds are water-soluble acridanes, enhancer coupled acridanes, bis and tris~acridanes as well as acridane-(1,2)- dioxetanes. Among the new (1,2)-dioxetanes are electron deficient group- containing dioxetanes and tethered bis-l,2-dioxetanes. The (1,2)-dioxetanes are useful as substrates for various enzymes. The acridanes can be admixed with an oxidizing agent. An aqueous buffer and , optionally, a stabilizer to form a substrate or reagent formulation useful for assaying, inter alia, HRP.

L'invention concerne de nouveaux composés chimioluminescents, stables dans les tampons aqueux, destinés à s'utiliser dans des dosages biologiques, qui comprennent des composés à base d'acridane et de (1,2)-dioxétanes. Parmi les nouveaux composés à base d'acridane on compte des acridanes hydrosolubles, des acridanes couplés aux substances de renforcement, des bis et tris-acridanes ainsi que des acridane-(1,2)-dioxétanes. Parmi les nouveaux (1,2)-dioxétanes on compte des dioxétanes contenant un groupe déficient en électrons et des bis-l,2-dioxétanes ancrés. Les (1,2)-dioxétanes sont utiles en tant que substrats pour diverses enzymes. Les acridanes peuvent être mélangées avec un agent d'oxydation. Un tampon aqueux et éventuellement un stabilisateur servent à former un substrat ou une formulation de réactifs utile pour doser, entre autres, HRP.

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