Nicotianamine synthase and gene encoding the same

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/54 (2006.01) C07K 1/36 (2006.01) C07K 16/40 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12P 17/10 (2006.01)

Patent

CA 2327261

A nicotianamine synthase is isolated and purified. Then the gene of this enzyme is cloned and the base sequence and amino acid sequence thereof are determined. This gene is employed in constructing plants, in particular, grass plants highly tolerant to iron deficiency. A nicotianamine synthase involved in the mugineic acid biosynthesis pathway; the amino acid sequence thereof; a gene encoding the same; a vector containing this gene; cells transformed by the vector; a process for producing nicotianamine by using the same; plants transformed by the gene encoding the nicotianamine synthase; and an antibody against the nicotianamine synthase.

On isole et on purifie une nicotianamine synthase. On clone ensuite le gène de cet enzyme et on détermine sa séquence de base et sa séquence aminoacide. On utilise ce gène afin de produire des plantes par génie génétique, en particulier, des plantes herbeuses extrêmement résistantes à la déficience en fer. Nicotianamine synthase jouant un rôle dans le trajet de la biosynthèse de l'acide muginéique; sa séquence aminoacide; gène la codant; vecteur contenant ce gène; cellules transformées par ce vecteur; procédé servant à produire de la nicotianamine au moyen de ce vecteur; plantes transformées par le gène codant cette nicotianamine synthase et anticorps contre cette nicotianamine synthase.

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