Nitrile hydratases from metagenome libraries

Details Nitrile hydratases from metagenome libraries Nitrile hydratases from metagenome libraries

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 9/88 (2006.01) C12N 15/52 (2006.01)

Patent

CA 2557476

The present invention is concerned with the preparation of novel nitrile hydratases. These latter are preferably obtained from nonculturable organisms by means of a PCR-based screening, in metagenome DNA libraries, using special degenerate primers.

La présente invention a trait à la préparation de nouvelles nitrile hydratases. Celles-ci sont, de préférence, obtenues à partir d'organismes non aptes à la culture par criblage basé sur la réaction en chaîne de la polymérase, dans des bibliothèques d'ADN métagénomique, au moyen d'amorces de dégradation spéciales.

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