C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12N 5/10 (2006.01) C12N 7/01 (2006.01) C12N 9/00 (2006.01) C12N 15/11 (2006.01) C12N 15/85 (2006.01) C12N 15/86 (2006.01)
Patent
CA 2315242
Novel double-stranded DNAs, expression vectors and methods for their use are provided in which the intracellular expression of the double-stranded DNAs alters the phenotype of a cell to determine the function of a gene of interest. The double-stranded DNAs encode a family of catalytic RNAs targeted to the mRNA of a gone of interest. Cleavage of the mRNA results in an altered cell phenotype from which the function of the product encoded by the mRNA is determined. The compositions find use in high-throughput screens to assign gene functions which eliminate the requirement of bacterial cloning.
L'invention concerne de nouveaux ADN double brin, des vecteurs d'expression, et leurs méthodes d'utilisation, l'expression intracellulaire de ces ADN double brin modifiant le phénotype d'une cellule de manière à déterminer la fonction d'un gène d'intérêt. Ces ADN double brin codent une famille d'ARN catalytiques ciblés vers l'ARN messager d'un gène d'intérêt. On obtient, par clivage de l'ARN messager, un phénotype cellulaire modifié, à partir duquel on peut déterminer la fonction du produit codé par cet ARN messager. Les compositions de la présente invention sont notamment utiles dans des cribles à haut rendement, permettant d'attribuer des fonctions géniques ne nécessitant aucun clonage bactérien.
Keck James G.
Kuo Sophia S.
Molony Jocelyn M.
Caprotec Bioanalytics Gmbh
Genetrace Systems Inc.
Smart & Biggar
LandOfFree
Non-bacterial cloning in delivery and expression of nucleic... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Non-bacterial cloning in delivery and expression of nucleic..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Non-bacterial cloning in delivery and expression of nucleic... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1904356