Non-competitive immunoassay for small analytes

Details Non-competitive immunoassay for small analytes Non-competitive immunoassay for small analytes

C - Chemistry, Metallurgy

12

N

C12N 15/13 (2006.01) C07K 16/42 (2006.01) C07K 16/44 (2006.01) G01N 33/53 (2006.01) G01N 33/542 (2006.01) G01N 33/68 (2006.01) G01N 33/94 (2006.01)

Patent

CA 2503509

The invention is directed to a non-competitive immunoassay for small analytes, wherein the analyte is reacted with two binding partners. The first binding partner binds to the analyte to form a complex between the first binding partner and the analyte, and the second binding partner binds to the complex formed by the first binding partner and the analyte. The resulting complex formed between the analyte and the binding partners is detected. The binding partners are proteins, such as antibodies including antibody fragments. The invention further relates to reagent pairs and test kits useful in the assays, as well as to the use of the reagent pairs and to a process for their preparation. Novel reagents and means for their preparation are also provided.

L'invention concerne un dosage immunologique non compétitif pour substances à analyser de taille réduite, selon lequel on fait réagir la substance à analyser avec deux partenaires de liaison. Le premier partenaire de liaison se lie à la substance à analyser pour former une complexe entre le premier partenaire de liaison et la substance à analyser, et le second partenaire de liaison se lie au complexe formé par le premier partenaire de liaison et la substance à analyser. On procède à la détection du complexe résultant, formé entre la substance à analyser et les partenaires de liaison. Les partenaires de liaison sont des protéines, telles que des anticorps, y compris des fragments d'anticorps. L'invention a également trait à des paires de réactifs et à des kits d'essai utiles pour les dosages, ainsi qu'à l'utilisation desdites paires de réactifs et à leur procédé de préparation. L'invention concerne enfin de nouveaux réactifs et des moyens permettant de préparer ces derniers.

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