Non-contact fluid transfer methods apparatus and uses thereof

Details Non-contact fluid transfer methods apparatus and uses thereof Non-contact fluid transfer methods apparatus and uses thereof

B - Operations, Transporting

01

L

B01L 3/02 (2006.01) B01L 3/00 (2006.01) G01N 21/11 (2006.01)

Patent

CA 2430422

Invention methods employ the use of acoustic waves to transfer small amounts of fluid in a non-contact manner. In invention methods, acoustic waves are propagated through a separated pool of a source fluid in such a manner that causes the ejection of a single micro-droplet from the surface of the pool. The droplet is ejected towards a target with sufficient force to provide for contact of the droplet with the target. Because the fluid is not contacted by any fluid transfer device such as a pipette, the opportunities for contamination are minimized. Invention methods may be employed to transfer fluids from an array of source sites to an array of target sites, thereby enabling the precision automation of a wide variety of procedures including screening, and synthesis procedures commomly used in biotechnology.

Cette invention concerne des procédés faisant intervenir des ondes acoustiques pour le transfert sans contact de petites volumes. On fait se propager des ondes acoustiques dans un réservoir distinct de liquide source de manière à provoquer l'éjection d'une micro-gouttelette unique à partir de la surface du réservoir. La gouttelette est éjectée vers une cible avec une force suffisante pour faire pénétrer cette gouttelette avec la cible. En l'absence de contact entre le liquide et un quelconque dispositif de transfert tel qu'une pipette, les risques de contamination sont réduits au minimum. Ces procédés peuvent s'utiliser pour transférer des liquides d'un ensemble de sites sources à un ensemble de sites cibles et rendent possible une automatisation de précision pour les techniques les plus diverses, criblage y compris, et pour des méthodes de synthèse couramment utilisées dans le domaine des biotechnologies.

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