Non-denaturing lysis reagent for use with...

G - Physics – 01 – N

Patent

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G01N 1/28 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2673314

The invention provides a lysis reagent and method for preparing a test sample for use in an assay, wherein the method yields a homogeneous lysis mixture suitable for use in automated pipetting systems without the need for a centrifugation step. The lysis reagent includes a glycol and non-specific animal immunoglobulins. Other aspects of the invention include related immunoassays and test kits.

La présente invention concerne un réactif de lyse et un procédé de préparation d'un échantillon d'essai destinés à être utilisés dans un dosage, selon lequel le procédé permet d'obtenir un mélange de lyse homogène apte à être utilisé dans un procédé de pipetage sans nécessiter une étape de centrifugation. Le réactif de lyse comporte un glycol et des immunoglobulines d'origine animale non spécifiques. Selon d'autres aspects, la présente invention concerne également des immunoessais et des trousses d'essai.

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