Non-destructive procedure for the isolation of dna from plants

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/10 (2006.01)

Patent

CA 2637831

The present invention relates to a method for obtaining DNA from a plant comprising collecting the root border cells from a growing root; and extracting DNA from the root border cells. The root border cells are contained in the root exudate of the growing root, which is growing in a medium, such as water, tissue culture medium or soil. Suitably, the root is part of a germinating seed, or the root of a seedling or the adventitious root of a tissue culture plant or plant part.

La présente invention concerne un procédé pour obtenir un ADN à partir d'une plante comprenant la collecte de cellules de bordure de racine à partir d'une racine en croissance ; et l'extraction d'ADN à partir des cellules de bordure de racine. Les cellules de bordure de racine sont contenues dans un exsudat de racine de la racine en croissance, qui pousse dans un milieu tel que l'eau, un milieu de culture de tissus ou un sol. De manière adéquate, la racine fait partie d'une graine en germination, ou de la racine d'un semis ou de la racine adventive d'une partie de plante ou d'une plante de culture de tissus.

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