Non-endogenous, constitutively activated versions of plant g...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/29 (2006.01) A01H 3/00 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C07K 14/705 (2006.01) C07K 19/00 (2006.01) C12N 15/82 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2449553

The invention relates to transmembrane receptors for which the endogenous ligand has not been identified, and specifically to a plant GPCR ("GCR1") that has been altered to establish constitutive activity of the receptor. In some embodiments, the altered versions of GCR1 are used for the direct identification of candidate compounds as receptor agonists, inverse agonists or partial agonists for use in, for example and not limitation, herbicidal relevance; germination; growth elongation; seed dormancy; and fruit and vegetable ripening and development. In some embodiments, altered versions of GCR1 are used to modulate physiological processes in a plant. The invention further relates to plants comprising constitutively activated non-endogenous GPCRs.

La présente invention concerne des récepteurs transmembranaires pour lesquels on n'a pas encore identifié le ligand endogène, et plus particulièrement un récepteur GPCR de végétal (GCR1) qui a été modifié pour établir une activité constitutive du récepteur. Pour certains modes de réalisation, les versions modifiées du GCR1 servent à l'identification directe de candidats composés servant d'agonistes des récepteurs, d'agonistes inverses ou d'agonistes partiels destinés, notamment et de façon non limitative, à la pertinence herbicide, la germination l'élongation de croissance la dormance des semences, ainsi qu'au mûrissement et au développement des fruits et légumes. Pour certains modes de réalisation, les versions modifiées du GCR1 servent à moduler des processus physiologiques dans une plante. L'invention concerne enfin des végétaux comprenant des GPCR non-endogènes consécutivement activés

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