Non-enzymatic immunohistochemical staining system and reagents

Details Non-enzymatic immunohistochemical staining system and reagents Non-enzymatic immunohistochemical staining system and reagents

G - Physics

01

N

167/37, 150/5

G01N 33/53 (2006.01) G01N 33/532 (2006.01) G01N 33/563 (2006.01) G01N 33/574 (2006.01) G01N 33/58 (2006.01)

Patent

CA 1269323

ABSTRACT OF THE DISCLOSURE A non-enzymatic method of immunohistochemical staining uses a bound electron transfer agent to achieve amplification of antibody binding to specific antigens of interest in a histology or cytology specimen. A preferred embodiment uses a bound phenazine, which is reduced with a soluble reducing agent, and which in turn reduces a tetrazolium salt, to precipitate a formazan dye at the site of primary antibody binding to the antigen of interest. Reagents and kits for use in the method are also provided.

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