Non-human animal model of systemic lupus erythematosus

A - Human Necessities – 01 – K

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Details

A01K 67/027 (2006.01) A61K 45/00 (2006.01) A61P 37/02 (2006.01) C07K 16/18 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2468415

The present invention is to provide a non-human animal model of systemic lupus erythematosus wherein generation of anti-double stranded DNA antibody and anti-single stranded antibody is induced, and that is made to spontaneously develop glomerulonephritis and arthritis, and a screening method for a therapeutic agent for systemic lupus erythematosus wherein the non-human animal model is used. Fc.gamma.RIIB deficient mouse that is not made to spontaneously develop autoimmune pathology although its autoantibody response is enhanced is backcrossed into C57BL/6J (B6) mouse for 12 generations to generate Fc.gamma.RIIB deficient B6 mouse, the Fc.gamma.RIIB deficient B6 male mouse is intercrossed with lpr/B6 female mouse, and thus obtained Fc.gamma.RIIB+/-/lpr+/- mice were further crossed to generate a mouse model of systemic lupus erythematosus.

L'invention concerne un animal modèle non humain du lupus érythémateux chronique souffrant d'un début spontané d'arthrite et de glomérulonéphrite par induction de la production d'un anticorps anti-ADN bicaténaire ou d'un anticorps anti-ADN monocaténaire, ainsi qu'un procédé de criblage de remède pour le lupus érythémateux chronique au moyen de cet animal. Des souris ne possédant pas Fc.gamma.RIIB, montrant une réaction autoanticorps accélérée, mais ne souffrant pas d'un début spontané de troubles pathogènes auto-immuns, sont rétro-croisées sur 12 générations avec des souris à C57BL/6J(B6) afin de construire des souris B6 ne possédant pas Fc.gamma.RIIB. Puis une souris mâle B6 ne possédant pas Fc.gamma.RIIB est croisée avec une souris femelle lpr/B6 et les animaux obtenus Fc.gamma.RIIB?+/-¿/lpr?+/-¿ sont croisés afin d'obtenir une souris modèle du lupus érythémateux chronique.

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