Non-invasive method for isolation and detection of fetal dna

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12Q 1/24 (2006.01) G01N 33/50 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01) G01N 33/80 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2068660

A method of isolating fetal nucleated cells, particularly fetal nucleated erythrocytes, from a maternal blood sample, by means of an antigen present on the cell surface of the fetal erythrocytes. A method of detecting fetal DNA of interest, which is a gene or gene portion associated with a disease or condition, a chromosomal abnormality or sex-specific DNA, in a maternal blood sample. The presence or absence, as well as the quantity of fetal DNA of interest in a maternal sample can be determined. The claimed method of detection can be used prenatally or postnatally and is particularly useful because it is noninvasive and can be carried out early in pregnancy.

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