Non-pcr based recombination of nucleic acids

Details Non-pcr based recombination of nucleic acids Non-pcr based recombination of nucleic acids

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12N 15/66 (2006.01)

Patent

CA 2406466

Described herein are methods for forming recombined nucleic acids which do not require the use of PCR for purposes of assembly or recombination. As such, the recombined nucleic acids are not subjected to size limitations or amplification of undesired errors. Moreover, the present invention allows recombination of sequences with low homology which would not occur if subjected to the high temperatures of PCR. Additionally, methods of forming proteins, screening assays, genetic pools, and recombined nucleic acids and proteins are provided herein.

L'invention concerne des procédés pour former des acides nucléiques de reconstruction qui ne nécessitent pas de réaction en chaîne de la polymérase (PCR) pour cet assemblage ou cette reconstruction. Ainsi, les acides nucléiques de reconstruction ne sont pas soumis à des limitations de taille ni à une amplification des erreurs indésirables. De plus, la présente invention permet de recombiner des séquences avec une faible homologie, ce qui ne serait pas possible avec les fortes températures de la PCR. En outre, l'invention concerne des procédés permettant de former des protéines, des dosages, des réserves de ressources génétiques et des protéines et des acides nucléiques de reconstruction.

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