Non-proteolytic method for the determination of analytes in...

G - Physics – 01 – N

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G01N 33/94 (2006.01)

Patent

CA 2723161

Methods that permit the rapid release of one or more analytes from head or body hair or other keratinized structures of an individual (who may previously have ingested one or more of the analytes) are provided. The methods can include contacting the keratinized structure with a reducing agent but not with a proteolytic agent. The methods can further include identification and quantification of the one or more analytes by known analytical techniques such as immunoassays. The described methods do not damage the analyte and do not cause harmful effects on a subsequently- used analyte detection probe (e.g., an antibody).

La présente invention concerne des procédés permettant une libération rapide d'un ou plusieurs analytes depuis des cheveux, des poils ou d'autres structures kératinisées d'un individu (qui peut avoir précédemment ingéré un ou plusieurs desdits analytes). Ces procédés comprennent la mise en contact de la structure kératinisée avec un agent réducteur, mais pas avec un agent protéolytique. Ces procédés peuvent encore comprendre l'identification et la quantification du ou des analytes par des techniques d'analyse connues telles que les immunodosages. Les procédés décrits n'endommagent pas l'analyte et n'ont pas d'effet nocif sur une sonde de détection d'analyte susceptible d'être utilisée ultérieurement (par exemple un anticorps).

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