Non-radioactive method for detecting a labelled segment and...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12Q 1/34 (2006.01) C12Q 1/42 (2006.01) G01N 33/53 (2006.01) G01N 33/535 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2155028

A non-radioactive method of detecting a ligand and antiligand complex labelled with alkaline phosphatase or a tracer having alkaline phosphatase conjugated thereto comprises reacting the complex with bromo-chloro-indolyl phosphate (BCIP), phenazine methosulfate (PMS) and dimethylthiazol diphenyl tetrazolium (MTT) and allowing the reaction to proceed to produce a colored formazan or a color change indicative of the presence of the labelled complex. A solution or composition of bromo-chloro-indolyl phosphate (BCIP), phonazine methosulfate (PMS) and dimethylthiazol diphenyl tetrazolium (MTT), as well as a test kit including the same, is also provided for carrying out the chromogenic method of detection

Un procédé non radioactif de détection d'un complexe ligand/antiligand marqué par la phosphatase alcaline ou un traceur auquel est conjuguée une phospatase alcaline, consiste à fare réagir le complexe avec du phosphate de bromo-chloro-indolyle (BCIP), du méthosulfate de phénazine (PMS) et du tétrazolium de diméthylthiazol diphényl (MTT), et à permettre à la réaction de se poursuivre pour produire un formazan coloré ou un changement de couleur indiquant la présence d'un complexe marqué. Une solution ou une composition de phosphate de bromo-chloro-indolyle (BCIP), de méthosulfate de phénazine (PMS) et de tétrazolium de diméthylthiazol diphényl (MTT) est également décrite, ainsi qu'un kit d'essai comprenant cette solution ou composition, et qui permet d'appliquer ce procédé de détection chromogène.

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