Non self-inactivating, expression targeted retroviral vectors

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/867 (2006.01) A61K 31/7088 (2006.01) A61K 35/76 (2006.01) A61K 48/00 (2006.01) C12N 7/01 (2006.01) C12N 7/04 (2006.01) C12N 15/11 (2006.01) C12N 15/48 (2006.01)

Patent

CA 2198210

The present invention relates to a retroviral vector undergoing promoter conversion comprising a 5'LTR region of the structure U3-R-U5; one or more sequences selected from coding and non-coding sequences; and a 3'LTR region comprising a completely or partially deleted U3 region wherein said deleted U3 region is replaced by a polylinker sequence, followed by the R and US region. Said retroviral vector undergoes promoter conversion and is useful as a gene transfer vehicle for targeted gene therapy.

La présente invention concerne un vecteur rétroviral subissant une conversion du promoteur et comprenant une région 5'LTR de la structure U3-R-U5; une ou plusieurs séquences choisies dans les séquences codantes et non codantes; et une région 3'LTR à région U3 est partiellement ou totalement supprimée et remplacée par une séquence de lieurs multisites, suivie par la région R et la région U5. Ledit vecteur rétroviral subit une conversion de promoteur(s) et est utile comme véhicule de transfert de gènes pour la thérapie génique ciblée.

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