Non-separation heterogenous assay for biological substance

Details Non-separation heterogenous assay for biological substance Non-separation heterogenous assay for biological substance

G - Physics

01

N

G01N 33/532 (2006.01) C12Q 1/34 (2006.01) C12Q 1/68 (2006.01) G01N 33/542 (2006.01) G01N 33/543 (2006.01) G01N 33/566 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2286414

This present invention is for a method referred to as non-separation heterogenous assay that greatly simplifies the detection, identification, measurement of concentration and activity of biological substances. It is based on the change of the label signal due to the uneven distribution of the label between surface and liquid in a vessel after completion of the reaction among reactants. The method involves the coating of a reactant (labeled or unlabeled) onto a surface, addition of a sample with or without a competitor labeled using a label tag or unlabeled. The change of the label signal can be directly measured.

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