Non-specific dna amplification

Details Non-specific dna amplification Non-specific dna amplification

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07K 14/08 (2006.01) C07K 14/535 (2006.01) C07K 14/54 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2067363

2067363 9105861 PCTABS00005 A method of non-specifically amplifying different-sequence fragments in a mixture of duplex DNA fragments is disclosed. The fragments are provided with end linkers, and the mixture is amplified by successive primer-initiated replication. Also disclosed is a method of cloning cDNA species which are homologous to a region of contiguous genomic DNA and are selected from a mixture of cDNA species.

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