Non-sulfonated cyanine dyes for labeling nucleosides and...

C - Chemistry – Metallurgy – 07 – H

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C07H 19/04 (2006.01) C07H 19/10 (2006.01) C07H 19/20 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2294979

A chemical compound of formula: (I), wherein R1 is selected from the group consisting of alkyl, aralkyl, and substituted alkyl groups; R3 is selected from the group consisting of H, PO3-2; P2O6-3; P3O9-4, and .alpha.-thio phosphates (PSO2-2; P2SO5-3; P3SO8-4); and .alpha.BH3- phosphates (P(BH3)O2-2, P2(BH3)O5-3, P3(BH3)O8-4); R4 is selected from the group consisting of H, lower alkyl, acyl, (CH2)pCOO(CH2)qCH3 wherein p is an integer from 0 to 4 and q is an integer from 0 to 4, and 5,6; 6,7; or 7,8- butadienyl, R5 is selected from the group consisting of H, lower alkyl, acyl, (CH2)pCOO(CH2)qCH3 wherein p is an integer from 0 to 4 and q is an integer from 0 to 4 and 5,6; 6,7; or 7,8- butadienyl; r is 1, 2 or 3 to form a second fused aromatic; X or Y are selected from the group consisting of O, S, C(R6)2, or N(R6), wherein R6 is preferably CH3 or a lower alkyl; and R3-0 - Sugar-Base is a nucleoside or nucleotide is disclosed.

Composé chimique de formule (I), dans laquelle R?1¿ est choisi dans le groupe comprenant des groupes alkyle substitué, alkyle, aralkyle; R?3¿ est chois dans le groupe consistant en H, PO¿3??-2¿; P¿2?O¿6??-3¿; P¿3?O¿9??-4¿, et .alpha.-thiophosphates (PSO¿2??-2¿; P¿2?SO¿5??-3¿; P¿3?SO¿8??-4¿); et .alpha.BH¿3??-¿ phosphates tels que (P(BH¿3?)O¿2??-2¿, P¿2?(BH¿3?)O¿5??-3¿, P¿3?(BH¿3?)O¿8??-4¿); R?4¿ est choisi dans le groupe constitué de H, alkyle inférieur, acyle, (CH¿2?)¿p?COO(CH¿2?)¿q?CH¿3?, p étant un entier de 0 à 4 et q étant un entier de 0 à 5, et 5,6; 6,7; ou 7,8-butadiényle; R?5¿ est choisi dans le groupe comprenant H, alkyle inférieur, acyle, (CH¿2?)¿p?COO(CH¿2?)¿q?CH¿3?, p étant un entier de 0 à 4 et q un entier de 0 à 4 et 5, 6; 6,7; ou 7,8-butadiényle; r vaut 1, 2, ou 3 pour former un deuxième aromatique fusionné; X ou Y sont choisis dans le groupe comprenant O, S, C(R?6¿)¿2?, ou N(R?6¿), R?6¿ étant de préférence CH¿3? ou alkyle inférieur; et R?3¿-0 -Sucre-Base est un nucléoside ou un nucléotide.

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