Nonhuman model animal nonresponsive to endotoxin and...

A - Human Necessities – 01 – K

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A01K 67/027 (2006.01) C12N 5/16 (2006.01) C12N 15/09 (2006.01) C12N 15/85 (2006.01) C12Q 1/02 (2006.01) G11C 13/00 (2006.01)

Patent

CA 2495727

It is intended to provide a nonhuman model animal nonresponsive to a bacterial component such as a lipoprotein, a lipopeptide, a peptidoglycan or an endotoxin which is useful in clarifying the function of an adaptor protein TIRAP of the TLR family; a promoter or an inhibitor for the response to a bacterial component with the use of the above nonhuman model animal which is nonresponsive to a bacterial component; a method of screening such a promoter or inhibitor; etc. A TIRAP-/- mouse lacking the function of the TIRAP gene on chromosome is constructed. Then it is confirmed that the responsibility to a ligand recognized by TLR2 such as a lipoprotein or a lipopeptide or a ligand recognized by TLR4 such as an endotoxin is specifically injured in this TIRAP- /- mouse.

L'invention concerne un animal modèle non humain ne réagissant pas à un composant bactérien, notamment à une lipoprotéine, à un lipopeptide, à un peptidoglycan ou à une endotoxine, utile dans la clarification de la fonction d'une protéine adaptatrice TIRAP de la famille TLR ; un promoteur ou un inhibiteur de la réponse d'un composant bactérien avec l'utilisation de l'animal modèle non humain susmentionné, ne réagissant pas à un composant bactérien. L'invention concerne une méthode de criblage d'un tel promoteur ou d'un tel inhibiteur etc. Une souris TIRAP?-¿/?-¿ ne présentant pas la fonction du gène TIRAP sur un chromosome est élaborée. Puis, on confirme que la réactivité pour un ligand reconnu par TLR2, notamment une lipoprotéine ou un lipopeptide, ou pour un ligand reconnu par TLR4, notamment une endotoxine, subit une lésion spécifique dans la souris TIRAP?-¿/?-¿.

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