C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2389268
Methods are provided for quantitatively determining the amount of polynucleotides in a sample. In one method, the distinct polynucleotide targets and standard polynucleotide targets are contacted with detectable probes and independently detectable single or double stranded complements to the standard targets under hybridization conditions. The hybridization pattern from the probe is compared to the hybridization pattern from the standard sequences to obtain quantitative information about the genetic profile of the labeled nucleic acid sample. Also provided are kits for the use of the methods.
L'invention concerne des procédés pour déterminer quantitativement la proportion de polynucléotides dans un échantillon. Selon un procédé, les cibles de polynucléotides distincts et les cibles de polynucléotides standard sont mis en contact avec des sondes détectables et des compléments à simple brin ou double brin, détectables de manière indépendante, des cibles standard, dans des conditions d'hybridation. Le motif d'hybridation de la sonde est comparé au motif d'hybridation des séquences standard pour permettre d'obtenir des informations quantitatives sur le profil génétique de l'échantillon d'acide nucléique marqué. L'invention concerne également des kits pour mettre en oeuvre lesdits procédés.
Arnold Lyle
Bedilion Tod
Bjel-Danes Erik
Lei Warren
Theriault Thomas P.
Ade & Company
Incyte Genomics Inc.
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