Normalization of samples for amplification reactions

Details Normalization of samples for amplification reactions Normalization of samples for amplification reactions

C - Chemistry, Metallurgy

07

H

C07H 21/04 (2006.01)

Patent

CA 2610861

The present invention provides oligonucleotide primers, compositions, methods, and kits for determining the amount of gDNA or the number of cells from which a cell lysate originated. The invention relies on detection and amplification of unique genomic sequences within the genome of an organism of interest to determine the amount of genomic nucleic acid in a sample. The invention can be used to normalize samples from particular cells, cell types, or organism, and provide an accurate basis for comparison of expression of genes across the samples.

La présente invention a trait à des amorces oligonucléotidiques, des compositions, des procédés, et des trousses pour la détermination de la quantité d'ADN génomique ou le nombre de cellules à partir desquelles un lysat cellulaire est dérivé. L'invention repose sur la détection et l'amplification de séquences génomiques uniques au sein du génome d'un organisme d'intérêt en vue de déterminer la quantité d'acide nucléique dans l'échantillons. L'invention peut être utilisée pour la normalisation d'échantillons provenant de cellules, de types de cellules, ou d'organisme particuliers, et fournissent une base précise pour la comparaison de l'expression de gènes à travers les échantillons.

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