C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01)
Patent
CA 2304895
The present invention relates generally to methods for producing normalized nucleic acid libraries in which each member of the library can be isolated with approximately equivalent probability. In particular, the present methods comprise subtractive hybridization of a nucleic acid library with haptenylated (e.g., biotinylated, avidinated or streptavidinated) nucleic acid molecules that are complementary to one or more of the nucleic acid molecules of the library, such that the variation in the abundances of the individual nucleic acid molecules in the library is reduced. The invention also relates to production of normalized nucleic acid libraries (particularly cDNA libraries) in which contaminating nucleic acid molecules have been reduced or eliminated, and to normalized nucleic acid libraries produced by such methods.
L'invention concerne en général des procédés de production de banques normalisées d'acides nucléiques, dans lesquelles chaque membre peut être isolé selon une probabilité approximativement équivalente. L'invention concerne notamment l'hybridation soustractive d'une banque d'acides nucléiques, comprenant des molécules d'acides nucléiques haptélynées (ex. biotinylées, avidinées ou streptavidinées), complémentaires d'une ou plusieurs desdites molécules d'acides nucléiques de la banque, de sorte que la variation de l'abondance des molécules d'acides nucléiques (notamment des banques d'ADNc) de la banque soit réduite. L'invention porte aussi sur la production de banques normalisées d'acides nucléiques dans lesquelles la contamination desdites molécules est réduite ou supprimée, et sur des banques normalisées d'acides nucléiques produites selon ces procédés.
Jessee Joel
Li Wu-Bo
Nisson Paul E.
Life Technologies Inc.
Life Technologies Corporation
Mbm Intellectual Property Law Llp
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