Novel alkaline pullulanase y having .alpha.-amylase...

C - Chemistry – Metallurgy – 12 – N

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195/123, 195/39,

C12N 9/44 (2006.01) C12N 1/20 (2006.01)

Patent

CA 2024952

A novel alkaline pullulanase Y having .alpha.-amylase activity; a microorganism producing said alkaline pullulanase Y; and a process for producing said alkaline pullulanase Y are disclosed. The alkaline pullulanase Y having .alpha.-amylase activity has its optimum pH at a higher alkaline range than conventional alkaline pullulanases and exhibits excellent stability over a wide pH range. Further, the alkaline pullulanase Y has strong resistance to almost all detergent components such as surfactants, chelating agents, proteases for detergents, and the like. Thus the alkaline pullulanase Y can advantageously be used as a detergent component.

Divulgation concernant une nouvelle pullulanase alcaline Y possédant une activité d'alpha-amylase, un microorganisme produisant ladite pullulanase alcaline Y, et un procédé pour la production de ladite pullulanase alcaline Y. La pullulanase alcaline Y possédant une activité d'alpha-amylase a un pH optimal dans une plage alcaline supérieure à celle des pullulanases alcalines classiques, et présente une excellente stabilité dans un grand intervalle de pH. De plus, la pullulanase alcaline Y résiste efficacement à presque tous les constituants de détergents, notamment les tensioactifs, les chélateurs, les protéases pour détergents, et autres. La pullulanase alcaline peut donc être employée avantageusement comme constituant dans les détergents.

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