Novel aminopeptidase derived from bacillus licheniformis,...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 9/56 (2006.01) C12N 1/20 (2006.01) C12N 9/48 (2006.01) C12N 15/57 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2451528

The present invention relates to a novel aminopeptidase derived from Bacillus licheniformis, a gene encoding the aminopeptidase, an expression vector containing the gene, a cell transformant transfected with the expression vector and a process for preparing a natural type protein using the enzyme. More particularly, the present invention relates to a gene encoding an aminopeptidase which is cloned and manufactured using the recombinant DNA technique, an expression vector containing the gene, a cell transformant transfected with the expression vector and a recombinant aminopeptidase which is necessary to produce a recombinant human growth hormone in a natural type protein and can be expressed in a high yield more stably and advantageously, compared with conventional methods for the purification.

La présente invention concerne une aminopeptidase dérivée du Bacillus licheniformis, un gène codant l'aminopeptidase, un vecteur d'expression contenant ledit gène, un transformant cellulaire transfecté à l'aide du vecteur d'expression et un procédé de préparation d'une protéine de type naturel faisant appel à ce dernier. La présente invention concerne notamment un gène codant l'aminopeptidase qui est clonée et fabriquée à l'aide de la technique de recombinaison de l'ADN, un vecteur d'expression contenant ledit gène, un transformant cellulaire transfecté par le vecteur d'expression et une aminopeptidase de recombinaison qui est nécessaire pour produire de l'hormone de croissance humaine de recombinaison dans une protéine de type naturel et qui peut être exprimée avec un rendement élevé de manière plus stable et plus avantageuse, comparé aux méthodes classiques de purification.

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