Novel amylases and uses thereof

Details Novel amylases and uses thereof Novel amylases and uses thereof

C - Chemistry, Metallurgy

12

N

C12N 15/56 (2006.01) C07K 14/38 (2006.01) C07K 16/14 (2006.01) C12N 9/30 (2006.01)

Patent

CA 2457850

The invention relates to newly identified polynucleotide sequences comprising genes that encode novel amylases isolated from Aspergillus niger. The invention features the full length nucleotide sequences of the novel genes, the cDNA sequences comprising the full length coding sequence of the novel amylases as well as the amino acid sequence of the full-length functional proteins and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein an amylase according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.

L'invention porte sur des séquences polynucléotidiques récemment identifiées qui comprennent des gènes de nouvelles amylases isolées d'Aspergillus niger. L'invention porte notamment sur des séquences nucléotidiques pleine longueur des nouveaux gènes, sur les séquences d'ADNc comprenant la séquence de codage pleine longueur des nouvelles amylases, ainsi que la séquence amino acide des protéines fonctionnelles pleine longueur et de leurs équivalents fonctionnels. L'invention porte également sur des procédés d'utilisation de ces enzymes dans des processus industriels et sur des procédés de diagnostic d'infections fongiques. L'invention a également trait à des cellules transformées par un polynucléotide de l'invention et à des cellules dans lesquelles une amylase de l'invention est génétiquement modifiée de façon à accroître ou réduire son activité et/ou son niveau d'expression.

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