Novel antigen constructs useful in the detection and...

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 19/00 (2006.01) A61K 39/21 (2006.01) C07K 14/16 (2006.01) G01N 33/564 (2006.01) C12N 15/49 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2300360

Isolated HIV-1 Group O env polypeptides obtained from the HIV-1 isolate HAM112 are claimed, as well as (a) antigen constructs comprising fusions of one or more of each of HIV-1 Group O env polypeptides and HIV-1 group M env polypeptide and (b) further antigen constructs containing additional Group O sequences and especially the gp41 IDR of isolate HAM112. Also claimed are polynucleotide sequences encoding the above, expression vectors comprising the same, host cells transformed thereby, and immunoassay methods and kits utilizing the antigen constructs of the invention.

L'invention porte sur des polypeptides isolés env du Groupe O du VIH obtenus à partir de l'isolat HAM112 du VIH, ainsi que (a) sur des produits de recombinaison d'antigènes qui comprennent la fusion d'un ou plusieurs de chacun des polypeptides env du groupe O du VIH avec un polypeptide env du Groupe M du VIH, et (b) sur des produits de recombinaison d'antigènes contenant des séquences supplémentaires du Groupe O et notamment la gp41 IDR de l'isolat HM112. L'invention porte également sur des séquences polynucléotidiques codant les produits de recombinaison des antigènes précités, les vecteurs d'expression les comprenant, les cellules hôtes transformées par ceux-ci, et l'invention porte de plus sur des procédés et des kits de dosage immunologique utilisant les produits de recombinaison des antigènes.

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