Novel artificial antigen presenting cells and uses therefor

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/85 (2006.01) A61K 48/00 (2006.01) C12N 15/11 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2568344

The invention relates to novel artificial antigen presenting cells (aAPCs). The aAPC comprises at least one stimulatory ligand and at least one costimulatory ligand where the ligands each specifically bind with a cognate molecule on a T cell of interest, thereby mediating expansion of the T cell. The aAPC of the invention can further comprise additional molecules useful for expanding a T cell of interest. The aAPC of the invention can be used as an ~off the shelf~ APC that can be readily designed to expand a T cell of interest. Also, the aAPC of the invention can be used identify the stimulatory, co-stimulatory, and any other factors that mediate growth and expansion of a T cell of interest. Thus, the present invention provides powerful tools for development of novel therapeutics where activation and expansion of a T cell can provide a benefit.

La présente invention concerne des cellules de présentation de nouveaux antigènes artificiels ou "aAPC" (pour "artificial Antigen Presenting Cell"). L'aAPC comprend au moins un ligand stimulant et au moins un ligand co-stimulant, chacun des ligands se liant spécifiquement à une molécule apparentée d'un lymphocyte T considéré, réalisant ainsi une médiation d'expansion du lymphocyte T. L'aAPC de l'invention peut en outre comprendre d'autres molécules convenant à l'expansion d'un lymphocyte T considéré. L'aAPC de l'invention peut s'utiliser comme APC déjà existant pouvant être facilement conçu pour réaliser l'expansion du lymphocyte T considéré. De la même façon, l'aAPC de l'invention peut s'utiliser pour identifier le stimulant, le co-stimulant et tous autres facteurs intervenant en médiateur pour la croissance et l'expansion d'un lymphocyte T considéré. L'invention propose ainsi de puissants outils de développement de nouveaux produits thérapeutiques dans la mesure où l'activation et l'expansion d'un lymphocyte T peut présenter de l'intérêt.

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