Novel bacillus thuringiensis strains active against...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/32 (2006.01) A01N 37/18 (2006.01) A01N 43/50 (2006.01) A01N 63/00 (2006.01) A01N 63/02 (2006.01) C07K 14/325 (2006.01) C12N 1/20 (2006.01)

Patent

CA 2151586

The invention is related to a novel biologically pure Bacillus thuringiensis (B.t.) strain active against lepidopteran and coleopteran pests which produces a bipyramidal crystal consisting essentially of a delta- endotoxin having a molecular weight of about 130,000 daltons and a rhomboidal crystal consisting essentially of two delta-endotoxins, each having a molecular weight of about 33,000 daltons, as well as spores, crystals, delta-endotoxins and/or mutants thereof. The invention also relates to insecticidal compositions obtainable therefrom. The invention further relates to methods of using the insecticidal compositions to control insect pest from the order Lepidoptera and/or Coleoptera. The invention also relates to isolated DNA sequences encoding the delta-endotoxins.

L'ivention se rapporte à une (des) nouvelle(s) souche(s) biologiquement pure(s) de Bacillus thuringiensis (B.t.) active(s) contre les parasites lépidoptères et coléoptères qui produit un cristal bipyramidal se composant essentiellement d'une endotoxine delta ayant une masse moléculaire d'environ 130 000 daltons et un cristal rhomboïdal se composant essentiellement de deux endotoxines delta, chacune ayant une masse moléculaire d'environ 33 000 daltons, ainsi que des spores, des cristaux, des endotoxines delta et/ou des mutants de cette souche. L'invention se rapporte également à des compositions insecticides pouvant être obtenues à partir de celles-ci, ainsi qu'à des procédés d'utilisation des compositions insecticides destinées à éliminer les parasites de l'ordre des lépidoptères et/ou des coléoptères. L'invention se rapporte aussi à des séquences d'ADN codant les endotoxines delta.

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