C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/32 (2006.01) A01N 63/00 (2006.01) C07H 21/00 (2006.01) C07K 14/325 (2006.01)
Patent
CA 2193625
The invention is related to a novel delta-endotoxins, each having a molecular weight of about 33,000. The delta-endotoxins are obtainable from a novel biologically pure Bacillus thuringiensis (B.t.) strains active against lepidopteran and coleopteran pests which produces a bipyramidal crystal consisting essentially of at least two delta-endotoxins having a molecular weight of about 130,000 daltons and a rhomboidal crystal consisting essentially of two delta-endotoxins, each having a molecular weight of about 33,000 daltons and/or mutants thereof. The invention also relates to insecticidal compositions obtainable therefrom. The invention further relates to methods of using the insecticidal compositions to control an insect pest(s) from the order Lepidoptera and/or Coleoptera. The invention also relates to isolated nucleic acid sequences encoding the delta-endotoxins and probes for detecting such nucleic acid sequences.
L'invention concerne de nouvelles delta-endotoxines possédant chacune un poids moléculaire d'environ 33.000. On obtient ces delta-endotoxines à partir de nouvelles souches biologiquement pures de Bacillus thuringiensis (B.t.) efficaces contre les parasites lépidoptères et coléoptères, et qui produisent un cristal bipyramidal constitué essentiellement d'au moins deux delta-endotoxines ayant un poids moléculaire d'environ 130.000 daltons et un cristal rhomboïdal constitué essentiellement de deux delta-endotoxines ayant chacune un poids moléculaire d'environ 33.000 daltons et/ou leurs mutants. L'invention concerne également des compositions insecticides que l'on peut obtenir à partir desdites souches. De plus, elle concerne des procédés d'utilisation desdites compositions insecticides, afin de lutter contre les insectes parasites lépidoptères et/ou coléoptères. Enfin, elle concerne des séquences isolées d'acide nucléique codant les delta-endotoxines, ainsi que des sondes de détection desdites séquences.
Adams Lee Fremont
Liu Chi-Li
Lufburrow Patricia A.
Thomas Michael David
Abbott Laboratories
Ogilvy Renault
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