Novel bmp-12-related proteins and methods of their manufacture

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) C07K 14/51 (2006.01)

Patent

CA 2727341

The invention provides novel BMP-12-related proteins, including methods of their manufacture. The proteins include substituted, truncated and substituted-truncated BMP-12-related proteins. The substituted BMP-12-related proteins contain a substitution at one or more oxidation-sensitive methionine residues with non- methionine residues, such as norleucine. The substituted BMP-12-related proteins exhibit normal bioactivity and enhanced resistance to oxidation, relative to the unsubstituted protein. The truncated BMP-12-related proteins exhibit enhanced activity.

L'invention concerne de nouvelles protéines apparentées à BMP-12, y compris des procédés pour les préparer. Les protéines comprennent des protéines apparentées à BMP-12 substituées, tronquées, et substituées et tronquées. Les protéines apparentées à BMP-12 substituées contiennent une substitution d'un ou plusieurs résidus méthionine sensibles à l'oxydation par des résidus non-méthionine, comme la norleucine. Les protéines apparentées à BMP-12 substituées présentent une activité biologique normale et une résistance accrue à l'oxydation, par rapport à la protéine non substituée. Les protéines apparentées à BMP-12 tronquées présentent une activité améliorée.

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