Novel chromogenous substrates for detecting bacterial hydrolase

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/34 (2006.01) C12Q 1/04 (2006.01)

Patent

CA 2325626

The invention concerns a chromogenous substrate for detecting the presence of at least one enzyme enzymatic activity. The invention also concerns a method for using such a substrate. The invention further concerns a method for identification based on such substrates and a device for implementing said identification method. The invention is characterised in that the substrate consists of at least two molecules, a first molecule consisting of a non-chromogenous marker part associated with at least a specific target part for the enzyme and a second molecule consisting of a non-chromogenous part, and the non-chromogenous marker part, once it is released, reacts with the second molecule to form a chromogenous molecule. The invention is particularly applicable in bacteriology.

La présente invention concerne un substrat chromogène permettant de détecter la présence de l'activité enzymatique d'au moins une enzyme. Elle concerne également un procédé d'utilisation d'un tel substrat. Elle concerne encore un procédé d'identification à base de tels substrats et un dispositif de mise en oeuvre de tel procédé d'identification. L'invention est caractérisée par le fait que le substrat est constitué d'au moins deux molécules, une première molécule constituée d'une partie marqueur non chromogène associée à au moins une partie spécifique cible de l'enzyme et une seconde molécule constituée d'une autre substance non chromogène, et que la partie marqueur non chromogène, une fois libérée, réagit avec la seconde molécule pour former une molécule chromogène. L'invention trouve une application préférentielle dans le domaine de la bactériologie.

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