Novel chromophores/fluorophores and methods for using the same

Details Novel chromophores/fluorophores and methods for using the same Novel chromophores/fluorophores and methods for using the same

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) A01K 67/00 (2006.01) C07K 14/435 (2006.01) C07K 16/18 (2006.01) C12N 15/63 (2006.01) C12P 21/02 (2006.01) C12Q 1/25 (2006.01) C12Q 1/68 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2454031

Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof, as well as the proteins encoded the same, are provided. The proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent Cnidarian, e.g., Anthozoan, species or are obtained from Anthozoan non-Pennatulacean (sea pen) species. Specific proteins of interest include the following specific proteins: hcriGFP; dendGFP; zoanRFP; scubGFP1; scubGFP2; rfloRFP; rfloGFP; mcavRFP; mcavGFP; cgigGFP; afraGFP; rfloGFP2; mcavGFP2; and mannFP. Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.

L'invention concerne des compositions d'acide nucléique codant de nouvelles chromo/fluoroprotéines et leur mutants, ainsi que des protéines codées par ces dernières. Ces protéines consistent en des protéines colorées et/ou fluorescentes, cette caractéristique étant induite par l'interaction entre deux ou plusieurs résidus de la protéine. Une autre caractéristique de ces protéines réside en leur obtention par des espèces non-bioluminescentes, telles que des Cnidaires ou, par exemple, des Anthozoaires ou des espèces Anthozoaires autres que Pennatulida. Des protéines spécifiques consistent en les protéines suivantes: hcriGFP, dendGFP, zoanRFP, scubGFP1, scubGFP2, rfloRFP, rfloGFP, mcavRFP, mcavGFP, cgigGFP, afraGFP, rfloGFP2, mcavGFP2, et mannFP. Les protéines recherchées consistent également en des protéines analogues à ces dernières ou en des mutants desdites protéines. Elle concerne également des fragments de ces acides nucléiques et les peptides codées par ceux-ci, ainsi que des anticorps contre ces protéines, des cellules transgéniques et des organismes transgéniques. Ces protéines et ces compositions d'acide nucléique peuvent être mises en application dans une variété de domaines différents. Elle concerne enfin des trousses contenant notamment ces compositions d'acide nucléique.

Affiliated with

Also associated with

Rating

Novel chromophores/fluorophores and methods for using the same does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Novel chromophores/fluorophores and methods for using the same, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Novel chromophores/fluorophores and methods for using the same will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1608936