Novel compositions comprising quaternary ammonium compounds...

A - Human Necessities – 61 – K

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A61K 9/127 (2006.01) A61K 48/00 (2006.01) C12N 15/87 (2006.01) C12N 15/88 (2006.01)

Patent

CA 2200952

The present invention provides compositions and methods which are useful for the introduction of polyanionic materials into cells. The compositions are mixtures of cationic compounds and neutral lipids which are typically formulated as liposomes. The cationic compounds are quaternary ammonium compounds wherein the nitrogen has two attached long chain alkyl groups, at least one of which is unsaturated. The methods for transfecting cells involve (a) contacting the polyanionic materials with the compositions above to form a polyanionic material-liposome complex, and (b) contacting the complex with the cells to be transfected.

La présente invention se rapporte à des compositions et des procédés permettant d'introduire des substances polyanioniques dans des cellules. Ces compositions sont des mélanges de composés cationiques et de lipides neutres généralement formulés sous forme de liposomes. Les composés cationiques sont des composés d'ammonium quaternaire dans lesquels l'azote renferme deux groupes alkyle à chaînes longues liés, dont au moins un est insaturé. Les procédés permettant de transfecter des cellules consistent à (a) mettre en contact les substances polyanioniques avec les compositions précitées pour former un complexe substance polyanionique/liposome, et (b) mettre ce complexe en contact avec les cellules à transfecter.

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