C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/71 (2006.01) C07K 14/105 (2006.01) C12N 9/88 (2006.01)
Patent
CA 2325601
The invention concerns a novel construct for expressing a gene coding for a recombinant protein of interest placed under the control of the Ptrp tryptophan operon promoter in a procaryotic host cell. The invention is characterised in that the construct comprises a nucleic sequence capable of inactivating the gene coding for a TnaA tryptophanase when said nucleic sequence is introduced in said host cell. The invention also concerns vectors containing said construct and host cells transformed by said vectors. The invention further concerns methods for producing said recombinant proteins using said novel constructs.
L'invention comprend une nouvelle construction pour l'expression d'un gène codant pour une protéine recombinante d'intérêt placé sous le contrôle du promoteur de l'opéron tryptophane Ptrp dans une cellule hôte procaryote, caractérisée en ce que la construction comprend une séquence nucléique capable d'inactiver le gène codant pour une tryptophanase TnaA lorsque ladite séquence nucléique est introduite dans ladite cellule hôte, des vecteurs contenant ladite construction et les cellules hôtes transformées par lesdits vecteurs. L'invention a également pour objet les procédés de production desdites protéines recombinantes utilisant ces nouvelles constructions.
Bonnefoy Jean-Yves
Chevalet Laurent
N'guyen Thien Ngoc
Robert Alain
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Pierre Fabre Medicament
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