Novel cryopreserved hepatocytes and process for doing same

Details Novel cryopreserved hepatocytes and process for doing same Novel cryopreserved hepatocytes and process for doing same

A - Human Necessities

01

N

A01N 1/02 (2006.01) A61K 35/407 (2006.01) A61L 27/38 (2006.01) C12M 3/00 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2223558

An artificial liver support system is described herein which comprises cryopreserved hepatocytes having an initial viability of 80-99 %. Further disclosed are hepatocytes cryopreserved by dispensing hepatocytes into freezing containers, freezing the containers from between minus 50 to minus 90 degrees Celcius, storing the containers in liquid or vapor nitrogen, thawing the cryopreserved hepatocytes when ready for use and removing residual cryoprotectant media.

L'invention concerne un système d'assistance de foie artificiel comprenant des hépatocytes cryoconservés présentant une viabilité initiale de 80 à 99 %. Elle concerne en outre des hépatocytes cryoconservés selon un procédé consistant à placer les hépatocytes dans des contenants de congélation, à congeler les contenants entre -50 et -90 ·C, à stocker les contenants dans de l'azote liquide ou de l'azote en phase vapeur, à décongeler les hépatocytes cryoconservés lorsqu'ils sont prêts à l'emploi et à éliminer les agents cryoprotecteurs résiduels.

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